Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054
Structural highlights
1xm5 is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
YBEY_ECOLI Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'-and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.[1][2][3][4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
↑Rasouly A, Davidovich C, Ron EZ. The heat shock protein YbeY is required for optimal activity of the 30S ribosomal subunit. J Bacteriol. 2010 Sep;192(18):4592-6. doi: 10.1128/JB.00448-10. Epub 2010 Jul 16. PMID:20639334 doi:http://dx.doi.org/10.1128/JB.00448-10
↑Davies BW, Kohrer C, Jacob AI, Simmons LA, Zhu J, Aleman LM, Rajbhandary UL, Walker GC. Role of Escherichia coli YbeY, a highly conserved protein, in rRNA processing. Mol Microbiol. 2010 Oct;78(2):506-18. doi: 10.1111/j.1365-2958.2010.07351.x. Epub, 2010 Sep 16. PMID:20807199 doi:http://dx.doi.org/10.1111/j.1365-2958.2010.07351.x
↑Jacob AI, Kohrer C, Davies BW, RajBhandary UL, Walker GC. Conserved bacterial RNase YbeY plays key roles in 70S ribosome quality control and 16S rRNA maturation. Mol Cell. 2013 Feb 7;49(3):427-38. doi: 10.1016/j.molcel.2012.11.025. Epub 2012, Dec 27. PMID:23273979 doi:http://dx.doi.org/10.1016/j.molcel.2012.11.025
↑Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA. The ybeY protein from Escherichia coli is a metalloprotein. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):959-63. Epub 2005 Oct 20. PMID:16511207 doi:10.1107/S1744309105031131
