2d00
Subunit F of V-type ATPase/synthase
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OverviewOverview
The crystal structure of subunit F of vacuole-type ATPase/synthase, (prokaryotic V-ATPase) was determined to of 2.2 A resolution. The subunit, reveals unexpected structural similarity to the response regulator, proteins that include the Escherichia coli chemotaxis response regulator, CheY. The structure was successfully placed into the low-resolution EM, structure of the prokaryotic holo-V-ATPase at a location indicated by the, results of crosslinking experiments. The crystal structure, together with, the single-molecule analysis using fluorescence resonance energy transfer, showed that the subunit F exhibits two conformations, a 'retracted' form, in the absence and an 'extended' form in the presence of ATP. Our results, postulated that the subunit F is a regulatory subunit in the V-ATPase.
About this StructureAbout this Structure
2D00 is a Single protein structure of sequence from Thermus thermophilus with CA as ligand. Active as H(+)-transporting two-sector ATPase, with EC number 3.6.3.14 Full crystallographic information is available from OCA.
ReferenceReference
Structure of a central stalk subunit F of prokaryotic V-type ATPase/synthase from Thermus thermophilus., Makyio H, Iino R, Ikeda C, Imamura H, Tamakoshi M, Iwata M, Stock D, Bernal RA, Carpenter EP, Yoshida M, Yokoyama K, Iwata S, EMBO J. 2005 Nov 16;24(22):3974-83. Epub 2005 Nov 10. PMID:16281059
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