2cps
SOLUTION NMR STRUCTURES OF THE MAJOR COAT PROTEIN OF FILAMENTOUS BACTERIOPHAGE M13 SOLUBILIZED IN SODIUM DODECYL SULPHATE MICELLES, 25 LOWEST ENERGY STRUCTURES
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OverviewOverview
The three-dimensional structure of the major coat protein of bacteriophage, M13, solubilized in detergent micelles, has been determined using, heteronuclear multidimensional NMR and restrained molecular dynamics. The, protein consists of two alpha-helices, running from residues 8 to 16 and, 25 to 45, respectively. These two helices are connected by a flexible and, distorted helical hinge region. The structural properties of the coat, protein make it resemble a flail, in which the hydrophobic helix (residues, 25 to 45) is the handle and the other, amphipathic, helix the swingle. In, this metaphor, the hinge region is the connecting piece of leather. The, mobility of the residues in the hinge region is likely to enable a smooth, transformation from the membrane-bound form, mimicked by the structure in, detergent micelles, into the structure in the mature phage. A specific, distribution of the residues over the surface of the two helices was, observed in the presented high-resolution structure of the membrane-bound, form of the major coat protein as well as in the structure in the mature, phage. All data suggest that this arrangement of residues is important for, the interactions of the protein with the membrane, for correct protein-DNA, and protein-protein interactions in the phage and for a proper growth of, the phage during the assembly process. By combining our findings with, earlier NMR results on the major coat protein in detergent micelles, we, were able to construct a model that addresses the role of specific, residues in the assembly process.
About this StructureAbout this Structure
2CPS is a Single protein structure of sequence from Enterobacteria phage mu. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of the M13 major coat protein in detergent micelles: a basis for a model of phage assembly involving specific residues., Papavoine CH, Christiaans BE, Folmer RH, Konings RN, Hilbers CW, J Mol Biol. 1998 Sep 18;282(2):401-19. PMID:9735296
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