1bk9
PHOSPHOLIPASE A2 MODIFIED BY PBPB
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OverviewOverview
The crystal structure of acidic phospholipase A2 (APLA2) from Agkistrodon, halys pallas covalently modified by p-bromo-phenacyl-bromide (pBPB) was, determined to a resolution of 2.0 A by an isomorphous difference Fourier, method with the native APLA2 structure as an initial model and refined to, a crystallographic R factor of 15.3%. The modified APLA2 structure is, remarkably similar to that of the native one. Least-squares superposition, of C alpha atoms of native and modified APLA2 results in a, root-mean-square coordinate deviation of 0.243 A. The p-bromo-phenacyl, group near the active site occupies a position similar to that in pBPB, modified bovine pancreatic PLA2. The inhibitor covalently bound to the NDI, atom of His48 fits well in the hydrophobic channel, forming extensive, ... [(full description)]
About this StructureAbout this Structure
1BK9 is a [Single protein] structure of sequence from [Gloydius halys] with CA, PBP and BU1 as [ligands]. Active as [[1]], with EC number [3.1.1.4]. Full crystallographic information is available from [OCA].
ReferenceReference
Structure of a snake venom phospholipase A2 modified by p-bromo-phenacyl-bromide., Zhao H, Tang L, Wang X, Zhou Y, Lin Z, Toxicon. 1998 Jun;36(6):875-86. PMID:9663694
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