1xey
Crystal structure of the complex of Escherichia coli GADA with glutarate at 2.05 A resolution
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OverviewOverview
Glutamate decarboxylase (GAD) is a pyridoxal enzyme that catalyzes the, conversion of L-glutamate into gamma-aminobutyric acid and carbon dioxide., The Escherichia coli enzyme exists as two isozymes, referred to as, GADalpha and GADbeta. Crystals of the complex of the recombinant isozyme, GADalpha with glutarate as a substrate analogue were grown in space group, R3, with unit-cell parameters a = b = 117.1, c = 196.4 angstroms. The, structure of the enzyme was solved by the molecular-replacement method and, refined at 2.05 angstroms resolution to an R factor of 15.1% (R(free) =, 19.9%). The asymmetric unit contains a dimer consisting of two subunits of, the enzyme related by a noncrystallographic twofold axis which is, perpendicular to and intersects a crystallographic threefold axis. The, dimers are related by a crystallographic threefold axis to form a hexamer., The active site of each subunit is formed by residues of the large domains, of both subunits of the dimer. The coenzyme pyridoxal phosphate (PLP), forms an aldimine bond with Lys276. The glutarate molecule bound in the, active site of the enzyme adopts two conformations with equal occupancies., One of the two carboxy groups of the glutarate occupies the same position, in both conformations and forms hydrogen bonds with the N atom of the main, chain of Phe63 and the side chain of Thr62 of one subunit and the side, chains of Asp86 and Asn83 of the adjacent subunit of the dimer., Apparently, it is in this position that the distal carboxy group of the, substrate would be bound by the enzyme, thus providing recognition of, glutamic acid by the enzyme.
About this StructureAbout this Structure
1XEY is a Single protein structure of sequence from Escherichia coli with ACT, PLP and GUA as ligands. Active as Glutamate decarboxylase, with EC number 4.1.1.15 Full crystallographic information is available from OCA.
ReferenceReference
Structure of Escherichia coli glutamate decarboxylase (GADalpha) in complex with glutarate at 2.05 angstroms resolution., Dutyshev DI, Darii EL, Fomenkova NP, Pechik IV, Polyakov KM, Nikonov SV, Andreeva NS, Sukhareva BS, Acta Crystallogr D Biol Crystallogr. 2005 Mar;61(Pt 3):230-5. Epub 2005, Feb 24. PMID:15735332
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