1irx
Crystal structure of class I lysyl-tRNA synthetase
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OverviewOverview
Lysyl-tRNA can be synthesized by both a class I (LysRS-I) and a class II, (LysRS-II) lysyl-tRNA synthetase. The crystal structure of LysRS-I from, Pyrococcus horikoshii at 2.6 A resolution reveals extensive similarity, with glutamyl-tRNA synthetase (GluRS). A comparison of the structures of, LysRS-I and LysRS-II in complex with lysine shows that both enzymes use, similar strategies for substrate recognition within unrelated active site, topologies. A docking model based upon the GluRS-tRNA complex suggests how, LysRS-I and LysRS-II can recognize the same molecular determinants in, tRNALys, as shown by biochemical results, while approaching the acceptor, helix of the tRNA from opposite sides.
About this StructureAbout this Structure
1IRX is a Single protein structure of sequence from Pyrococcus horikoshii with ZN as ligand. Active as Lysine--tRNA ligase, with EC number 6.1.1.6 Full crystallographic information is available from OCA.
ReferenceReference
Functional convergence of two lysyl-tRNA synthetases with unrelated topologies., Terada T, Nureki O, Ishitani R, Ambrogelly A, Ibba M, Soll D, Yokoyama S, Nat Struct Biol. 2002 Apr;9(4):257-62. PMID:11887185
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