1bk5
KARYOPHERIN ALPHA FROM SACCHAROMYCES CEREVISIAE
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OverviewOverview
Selective nuclear import is mediated by nuclear localization signals, (NLSs) and cognate transport factors known as karyopherins or importins., Karyopherin alpha recognizes "classical" monopartite and bipartite NLSs., We report the crystal structure of a 50 kDa fragment of the 60 kDa yeast, karyopherin alpha, in the absence and presence of a monopartite NLS, peptide at 2.2 A and 2.8 A resolution, respectively. The structure shows a, tandem array of ten armadillo repeats, organized in a right-handed, superhelix of helices. Binding of the NLS peptide occurs at two sites, within a helical surface groove that is lined by conserved residues. The, structure reveals the determinants of NLS specificity and suggests a model, for the recognition of bipartite NLSs.
About this StructureAbout this Structure
1BK5 is a Single protein structure of sequence from Saccharomyces cerevisiae with CO as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystallographic analysis of the recognition of a nuclear localization signal by the nuclear import factor karyopherin alpha., Conti E, Uy M, Leighton L, Blobel G, Kuriyan J, Cell. 1998 Jul 24;94(2):193-204. PMID:9695948
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