2hfg
Crystal structure of hBR3 bound to CB3s-Fab
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OverviewOverview
BR3, which is expressed on all mature B cells, is a specific receptor for, the B-cell survival and maturation factor BAFF (B-cell-activating factor, belonging to the tumor necrosis factor [TNF] family). In order to, investigate the consequences of targeting BR3 in murine models and to, assess the potential of BR3 antibodies as human therapeutics, synthetic, antibody phage libraries were employed to identify BAFF-blocking, antibodies cross-reactive to murine and human BR3, which share 52%, identity in their extracellular domains. We found an antibody, CB1, which, exhibits muM affinity for murine BR3 and very weak affinity for the human, receptor. CB3s, an affinity-matured variant of CB1, has sub-nM affinity, for BR3 from both species. Alanine scanning and crystallographic, structural analysis of the CB3s/BR3 complex reveal that CB3s mimics BAFF, by interacting with a similar region of the BR3 surface. Despite this, similarity in binding epitopes, CB1 variants antagonize BAFF-dependent, human B-cell proliferation in vitro and are effective at reducing murine, B-cell populations in vivo, showing significant promise as therapeutics, for human B-cell-mediated diseases.
DiseaseDisease
Known disease associated with this structure: Kappa light chain deficiency OMIM:[147200]
About this StructureAbout this Structure
2HFG is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Synthetic anti-BR3 antibodies that mimic BAFF binding and target both human and murine B cells., Lee CV, Hymowitz SG, Wallweber HJ, Gordon NC, Billeci KL, Tsai SP, Compaan DM, Yin J, Gong Q, Kelley RF, DeForge LE, Martin F, Starovasnik MA, Fuh G, Blood. 2006 Nov 1;108(9):3103-11. Epub 2006 Jul 13. PMID:16840730
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