1tz5
[pNPY19-23]-hPP bound to DPC Micelles
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OverviewOverview
Neuropeptide Y (NPY) and the pancreatic polypeptide (PP) are members of, the neuropeptide Y family of hormones. They bind to the Y receptors with, very different affinities: Whereas PP is highly selective for the Y(4), receptor, NPY displays highest affinites for Y(1), Y(2), and Y(5) receptor, subtypes. Introducing the NPY segment 19-23 into PP leads to an increase, in affinity at the Y(1) and Y(2) receptor subtypes whereas the exchange of, this segment from PP into NPY leads to a large decrease in affinity at all, receptor subtypes. PP displays a very stable structure in solution, with, the N terminus being back-folded onto the C-terminal alpha-helix (the, so-called PP-fold). The helix of NPY is less stable and the N terminus is, freely diffusing in solution. The exchange of this segment, however, does, not alter the PP-fold propensities of the chimeric peptides in solution., The structures of the phospholipid micelle-bound peptides serving to mimic, the membrane-bound species display segregation into a more flexible, N-terminal region and a well-defined alpha-helical region. The, introduction of the [19-23]-pNPY segment into hPP leads to an N-terminal, extension of the alpha-helix, now starting at Pro(14) instead of Met(17)., In contrast, a truncated helix is observed in [(19)(-)(23)hPP]-pNPY, starting at Leu(17) instead of Ala(14). All peptides display moderate, binding affinities to neutral membranes (K(assoc) in the range of 1.7 to, 6.8 x 10(4) mol(-)(1) as determined by surface plasmon resonance) with the, differences in binding being most probably related to the exchange of, Arg-19 (pNPY) by Glu-23 (hPP). Differences in receptor binding properties, between the chimeras and their parental peptides are therefore most likely, due to changes in the conformation of the micelle-bound peptides.
DiseaseDisease
Known disease associated with this structure: Neutral lipid storage disease with myopathy OMIM:[609059]
About this StructureAbout this Structure
1TZ5 is a Single protein structure of sequence from Homo sapiens and sus scrofa with NH2 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Strongly altered receptor binding properties in PP and NPY chimeras are accompanied by changes in structure and membrane binding., Lerch M, Kamimori H, Folkers G, Aguilar MI, Beck-Sickinger AG, Zerbe O, Biochemistry. 2005 Jun 28;44(25):9255-64. PMID:15966750
Page seeded by OCA on Mon Nov 12 19:29:42 2007