8ghf

cryo-EM structure of hSlo1 in plasma membrane vesiclescryo-EM structure of hSlo1 in plasma membrane vesicles

Structural highlights 8ghf is a 4 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , , , , Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function KCMA1_HUMAN Publication Abstract from PubMed Integral membrane protein structure determination traditionally requires extraction from cell membranes using detergents or polymers. Here, we describe the isolation and structure determination of proteins in membrane vesicles derived directly from cells. Structures of the ion channel Slo1 from total cell membranes and from cell plasma membranes were determined at 3.8 A and 2.7 A resolution, respectively. The plasma membrane environment stabilizes Slo1, revealing an alteration of global helical packing, polar lipid, and cholesterol interactions that stabilize previously unresolved regions of the channel and an additional ion binding site in the Ca(2+) regulatory domain. The two methods presented enable structural analysis of both internal and plasma membrane proteins without disrupting weakly interacting proteins, lipids, and cofactors that are essential to biological function. Membrane protein isolation and structure determination in cell-derived membrane vesicles.,Tao X, Zhao C, MacKinnon R Proc Natl Acad Sci U S A. 2023 May 2;120(18):e2302325120. doi: , 10.1073/pnas.2302325120. Epub 2023 Apr 25. PMID:37098056 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 8ghf, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image