1fq1
CRYSTAL STRUCTURE OF KINASE ASSOCIATED PHOSPHATASE (KAP) IN COMPLEX WITH PHOSPHO-CDK2
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OverviewOverview
The CDK-interacting protein phosphatase KAP dephosphorylates, phosphoThr-160 (pThr-160) of the CDK2 activation segment, the site of, regulatory phosphorylation that is essential for kinase activity. Here we, describe the crystal structure of KAP in association with pThr-160-CDK2, representing an example of a protein phosphatase in complex with its, intact protein substrate. The major protein interface between the two, molecules is formed by the C-terminal lobe of CDK2 and the C-terminal, helix of KAP, regions remote from the kinase-activation segment and the, KAP catalytic site. The kinase-activation segment interacts with the, catalytic site of KAP almost entirely via the phosphate group of pThr-160., This interaction requires that the activation segment is unfolded and, drawn away from the kinase molecule, inducing a conformation of CDK2, similar to the activated state observed in the CDK2/cyclin A complex.
About this StructureAbout this Structure
1FQ1 is a Protein complex structure of sequences from Homo sapiens with MG and ATP as ligands. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Full crystallographic information is available from OCA.
ReferenceReference
Phosphoprotein-protein interactions revealed by the crystal structure of kinase-associated phosphatase in complex with phosphoCDK2., Song H, Hanlon N, Brown NR, Noble ME, Johnson LN, Barford D, Mol Cell. 2001 Mar;7(3):615-26. PMID:11463386
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