1cx0

OverviewOverview

The self-cleaving ribozyme of the hepatitis delta virus (HDV) is the only, catalytic RNA known to be required for the viability of a human pathogen., We obtained crystals of a 72-nucleotide, self-cleaved form of the genomic, HDV ribozyme that diffract X-rays to 2.3 A resolution by engineering the, RNA to bind a small, basic protein without affecting ribozyme activity., The co-crystal structure shows that the compact catalytic core comprises, five helical segments connected as an intricate nested double pseudoknot., The 5'-hydroxyl leaving group resulting from the self-scission reaction is, buried deep within an active-site cleft produced by juxtaposition of the, helices and five strand-crossovers, and is surrounded by biochemically, important backbone and base functional groups in a manner reminiscent of, protein enzymes.

About this StructureAbout this Structure

1CX0 is a Single protein structure of sequence from Hepatitis delta virus with MG as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Crystal structure of a hepatitis delta virus ribozyme., Ferre-D'Amare AR, Zhou K, Doudna JA, Nature. 1998 Oct 8;395(6702):567-74. PMID:9783582

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