1nh0
1.03 A structure of HIV-1 protease: inhibitor binding inside and outside the active site
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OverviewOverview
The X-ray structure of a complex of HIV-1 protease (PR) with a, phenylnorstatine inhibitor Z-Pns-Phe-Glu-Glu-NH(2) has been determined at, 1.03 A, the highest resolution so far reported for any HIV PR complex. The, inhibitor shows subnanomolar K(i) values for both the wild-type PR and the, variant representing one of the most common mutations linked to resistance, development. The structure comprising the phenylnorstatine moiety of, (2R,3S)-chirality displays a unique pattern of hydrogen bonding to the two, catalytic aspartate residues. This high resolution makes it possible to, assess the donor and acceptor relations of this hydrogen bonding and to, indicate a proton shared by the two catalytic residues. A structural, mechanism for the unimpaired inhibition of the protease Val82Ala mutant is, also suggested, based on energy calculations and analyses.
About this StructureAbout this Structure
1NH0 is a Single protein structure of sequence from Human immunodeficiency virus 1 with SO4, NH2 and BME as ligands. Active as HIV-1 retropepsin, with EC number 3.4.23.16 Full crystallographic information is available from OCA.
ReferenceReference
A phenylnorstatine inhibitor binding to HIV-1 protease: geometry, protonation, and subsite-pocket interactions analyzed at atomic resolution., Brynda J, Rezacova P, Fabry M, Horejsi M, Stouracova R, Sedlacek J, Soucek M, Hradilek M, Lepsik M, Konvalinka J, J Med Chem. 2004 Apr 8;47(8):2030-6. PMID:15056001
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