6drv

Beta-galactosidaseBeta-galactosidase

6drv, resolution 2.20Å Show:Asymmetric Unit Biological Assembly Structural highlights 6drv is a 4 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: lacZ, b0344, JW0335 (ECOLI) Activity: Beta-galactosidase, with EC number 3.2.1.23 Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2A structure of ss-galactosidase in approximately 55h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud. cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.,Cianfrocco MA, Lahiri I, DiMaio F, Leschziner AE J Struct Biol. 2018 Jun 1. pii: S1047-8477(18)30138-2. doi:, 10.1016/j.jsb.2018.05.014. PMID:29864529 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.