Catabolite control protein

Function

Catabolite control protein (CcpA) or glucose-resistant amylase regulator is a regulator of carbon metabolism in gram-positive bacteria. When high concentrations of glucose-6 phosphate or fructose-1,6-diphosphate are present in the cell, they phosphorylate proteins HPr or Crh which interact with CcpA. The latter binds to DNA operator to modulate transcription.^[1]

Structural highlights

CcpA phosphorylation activity is carried out by a (PDB entry 2fep).^[2] ^[3]

3D structures of catabolite control protein

Catabolite control protein 3D structures

Structure of catabolite control protein (blue) complex with Hpr (green) and sulfate (PDB entry 2fep)

Drag the structure with the mouse to rotate

ReferencesReferences

↑ Iyer R, Baliga NS, Camilli A. Catabolite control protein A (CcpA) contributes to virulence and regulation of sugar metabolism in Streptococcus pneumoniae. J Bacteriol. 2005 Dec;187(24):8340-9. PMID:16321938 doi:http://dx.doi.org/10.1128/JB.187.24.8340-8349.2005
↑ Deutscher J, Reizer J, Fischer C, Galinier A, Saier MH Jr, Steinmetz M. Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis. J Bacteriol. 1994 Jun;176(11):3336-44. PMID:8195089
↑ Fujita Y, Miwa Y, Galinier A, Deutscher J. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol Microbiol. 1995 Sep;17(5):953-60. PMID:8596444

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

[1] Iyer R, Baliga NS, Camilli A. Catabolite control protein A (CcpA) contributes to virulence and regulation of sugar metabolism in Streptococcus pneumoniae. J Bacteriol. 2005 Dec;187(24):8340-9. PMID:16321938 doi:http://dx.doi.org/10.1128/JB.187.24.8340-8349.2005

[2] Deutscher J, Reizer J, Fischer C, Galinier A, Saier MH Jr, Steinmetz M. Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis. J Bacteriol. 1994 Jun;176(11):3336-44. PMID:8195089

[3] Fujita Y, Miwa Y, Galinier A, Deutscher J. Specific recognition of the Bacillus subtilis gnt cis-acting catabolite-responsive element by a protein complex formed between CcpA and seryl-phosphorylated HPr. Mol Microbiol. 1995 Sep;17(5):953-60. PMID:8596444

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