1emo
NMR STUDY OF A PAIR OF FIBRILLIN CA2+ BINDING EPIDERMAL GROWTH FACTOR-LIKE DOMAINS, 22 STRUCTURES
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OverviewOverview
The nuclear magnetic resonance structure of a covalently linked pair of, calcium-binding (cb) epidermal growth factor-like (EGF) domains from human, fibrillin-1, the protein defective in the Marfan syndrome, is described., The two domains are in a rigid, rod-like arrangement, stabilized by, interdomain calcium binding and hydrophobic interactions. We propose a, model for the arrangement of fibrillin monomers in microfibrils that, reconciles structural and antibody binding data, and we describe a set of, disease-causing mutations that provide the first clues to the specificity, of cbEFG interactions. The residues involved in stabilizing the domain, linkage are highly conserved in fibrillin, fibulin, thrombomodulin, and, the low density lipoprotein receptor. We propose that the relative, orientation of tandem cbEGF domains in these proteins is similar, but that, in others, including Notch, pairs adopt a completely different, conformation.
About this StructureAbout this Structure
1EMO is a Single protein structure of sequence from Homo sapiens with CA as ligand. Structure known Active Sites: CA1 and CA2. Full crystallographic information is available from OCA.
ReferenceReference
Solution structure of a pair of calcium-binding epidermal growth factor-like domains: implications for the Marfan syndrome and other genetic disorders., Downing AK, Knott V, Werner JM, Cardy CM, Campbell ID, Handford PA, Cell. 1996 May 17;85(4):597-605. PMID:8653794
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