2zao

Crystal structure of mouse SKD1/VPS4B ADP-formCrystal structure of mouse SKD1/VPS4B ADP-form

FunctionFunction

[VPS4B_MOUSE] Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (By similarity).^[1] ^[2]

About this StructureAbout this Structure

2zao is a 1 chain structure with sequence from Lk3 transgenic mice. Full crystallographic information is available from OCA.

ReferenceReference

^{[xtra 1]}

↑ Inoue M, Kamikubo H, Kataoka M, Kato R, Yoshimori T, Wakatsuki S, Kawasaki M. Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B. Traffic. 2008 Dec;9(12):2180-9. Epub 2008 Oct 8. PMID:18796009 doi:10.1111/j.1600-0854.2008.00831.x

↑ Scheuring S, Bodor O, Rohricht RA, Muller S, Beyer A, Kohrer K. Cloning, characterisation, and functional expression of the Mus musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and the yeast VPS4 genes are orthologues and involved in intracellular protein trafficking. Gene. 1999 Jun 24;234(1):149-59. PMID:10393249
↑ Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, Miwako I, Ohashi M, Ohsumi M, Ohsumi Y. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000 Feb;11(2):747-63. PMID:10679028

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OCA

[3] Inoue M, Kamikubo H, Kataoka M, Kato R, Yoshimori T, Wakatsuki S, Kawasaki M. Nucleotide-dependent conformational changes and assembly of the AAA ATPase SKD1/VPS4B. Traffic. 2008 Dec;9(12):2180-9. Epub 2008 Oct 8. PMID:18796009 doi:10.1111/j.1600-0854.2008.00831.x

[1] Scheuring S, Bodor O, Rohricht RA, Muller S, Beyer A, Kohrer K. Cloning, characterisation, and functional expression of the Mus musculus SKD1 gene in yeast demonstrates that the mouse SKD1 and the yeast VPS4 genes are orthologues and involved in intracellular protein trafficking. Gene. 1999 Jun 24;234(1):149-59. PMID:10393249

[2] Yoshimori T, Yamagata F, Yamamoto A, Mizushima N, Kabeya Y, Nara A, Miwako I, Ohashi M, Ohsumi M, Ohsumi Y. The mouse SKD1, a homologue of yeast Vps4p, is required for normal endosomal trafficking and morphology in mammalian cells. Mol Biol Cell. 2000 Feb;11(2):747-63. PMID:10679028

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