2gmg
Solution NMR Structure of protein PF0610 from Pyrococcus furiosus, Northeast Structural Genomics Consortium Target PfG3
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OverviewOverview
PF0610, a protein from the hyperthermophile Pyrococcus furiosus, has, homologues only in other archaeal species and in three species of, Fe(III)-reducing bacteria. It is thought to have a helix-turn-helix (HTH), domain at the N-terminus and possesses two CXXC motifs characteristic of, metal binding proteins. We have determined the solution structure of the, Zn-bound protein using NMR. PF0610 is a novel winged helix-turn-helix, (wHTH) protein with a rubredoxin-like Zn ribbon as its W1 segment. In, addition, it possesses a large number of basic residues on its surface., Clusters of basic residues can be found on both helix H3 and the, metal-binding loops of W1, suggesting that it might be a DNA-binding, protein. Accordingly, gel shift assays using both linear and circular DNA, showed that PF0610 does bind DNA, at least in a sequence-independent, fashion. Modeling the PF0610-DNA interaction based on other wHTH, protein-DNA structures revealed that besides helix H3, basic residues, around the second CXXC motif in the metal-binding loop could make, extensive contacts with DNA. However, the bulkiness of the W1 region, implies that the DNA conformation may be distorted upon PF0610 binding., PF0610 is the first protein known to have a Zn ribbon-embedded wHTH fold, and, as such, has potential roles both as a metal-dependent transcription, regulator and as a component of the chromosome packing system in P., furiosus. The discovery of this novel structure represents the addition of, another branch to the winged HTH protein family and could contribute to, our understanding of transcription regulatory processes in P. furiosus.
About this StructureAbout this Structure
2GMG is a Single protein structure of sequence from Pyrococcus furiosus dsm 3638. Full crystallographic information is available from OCA.
ReferenceReference
PF0610, a novel winged helix-turn-helix variant possessing a rubredoxin-like Zn ribbon motif from the hyperthermophilic archaeon, Pyrococcus furiosus., Wang X, Lee HS, Sugar FJ, Jenney FE Jr, Adams MW, Prestegard JH, Biochemistry. 2007 Jan 23;46(3):752-61. PMID:17223696
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