Muconate cycloisomerase (MCI) catalyzes the formation of muconolactone from cis,cis-muconate. It is part of the B-ketoadipate pathway in the metabolism of aromatic compounds[1]. MCI breaks down lignin-derived catechol and protocatechuate.
- Chloromuconate cycloisomerase catalyzes the reversible conversion of 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate to cis,cis-butadiene-1,2,4-tricarboxylate [2].
- 3-carboxy-cis,cis-muconate cycloisomerase catalyzes the reversible conversion of 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate to 3-chloro-cis,cis-muconate[3].
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3D Structures of muconate cycloisomerase3D Structures of muconate cycloisomerase
Updated on 10-November-2021
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- Chloro-MCI
- 2chr – MCI – Cupriavidsua necator
- 1nu5 - MCI – Pseudomonas
- 4m0x – MCI – Rhodococcus opacus
- MCI
- 1muc, 1bkh – PbMCI – Pseudomonas putida
- 3muc, 2muc, 1f9c – PbMCI (mutant)
- 2oqy, 3fyy – OiMCI – Oceanobacillus iheyensis
- 3i4k – MCI – Corynebacterium glutamicum
- 3i6e – MCI – Ruegeria pomeroyi
- 3i6t – MCI – Jannaschia
- 3my9 – MCI – Azorhizobium caulinodans
- 2zad – TmMCI – Thermotoga maritima
- 3ct2 - PfMCI – Pseudomonas fluorescens
- 3dg3 – MsMCI - Mycobacterium smegmatis
- 3fcp – MCI – Klebsiella pneumoniae
- MCI complexes
- 3es7, 3es8 – OiMCI + Mg + malate
- 3gd6 – OiMCI + phosphate
- 3hpf – OiMCI (mutant) + Mg + galactarate
- 3dg6, 3dg7 – MsMCI + muconolactone
- 3dgb, 3fj4 - PfMCI + muconolactone
- 3-carboxy-cis, cis-MCI
- 1q5n – MCI – Acinetobacter calcoaceticus
- 1re5 - PbMCI
- 1jof – MCI – Neurospora crassa
- 2fel, 2fen – MCI – Agrobacterium tumefaciens
- Dipeptide epimerase
- 3iji, 3ijl, 3ijq– MCI + dipeptide – Bacterioides thetaiotaomicron
- 3deq, 3der, 3des – TmMCI + dipeptide
- 3dfy – TmMCI
- 3q45, 3q4d – MCI + Mg + dipeptide – Cytophaga hutchinsonii
- 3rit - MCI + Mg + dipeptide – Methylococcus capsulatus
- 3jva – EfMCI + Mg – Enterococcus faecalis
- 3jw7, 3jzu, 3k1g, 3kum – EfMCI + Mg + dipeptide
ReferencesReferences
- ↑ Vollmer MD, Hoier H, Hecht HJ, Schell U, Groning J, Goldman A, Schlomann M. Substrate specificity of and product formation by muconate cycloisomerases: an analysis of wild-type enzymes and engineered variants. Appl Environ Microbiol. 1998 Sep;64(9):3290-9. PMID:9726873
- ↑ Schmidt E, Knackmuss HJ. Chemical structure and biodegradability of halogenated aromatic compounds. Conversion of chlorinated muconic acids into maleoylacetic acid. Biochem J. 1980 Oct 15;192(1):339-47. PMID:7305906
- ↑ Ornston LN. The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida. II. Enzymes of the protocatechuate pathway. J Biol Chem. 1966 Aug 25;241(16):3787-94. PMID:5916392
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