3v6f
Crystal Structure of an anti-HBV e-antigen monoclonal Fab fragment (e6), unboundCrystal Structure of an anti-HBV e-antigen monoclonal Fab fragment (e6), unbound
Structural highlights
Publication Abstract from PubMedChronic hepatitis B virus (HBV) infection afflicts millions worldwide with cirrhosis and liver cancer. HBV e-antigen (HBeAg), a clinical marker for disease severity, is a nonparticulate variant of the protein (core antigen, HBcAg) that forms the building-blocks of capsids. HBeAg is not required for virion production, but is implicated in establishing immune tolerance and chronic infection. Here, we report the crystal structure of HBeAg, which clarifies how the short N-terminal propeptide of HBeAg induces a radically altered mode of dimerization relative to HBcAg ( approximately 140 degrees rotation), locked into place through formation of intramolecular disulfide bridges. This structural switch precludes capsid assembly and engenders a distinct antigenic repertoire, explaining why the two antigens are cross-reactive at the T cell level (through sequence identity) but not at the B cell level (through conformation). The structure offers insight into how HBeAg may establish immune tolerance for HBcAg while evading its robust immunogenicity. Antigenic switching of hepatitis B virus by alternative dimerization of the capsid protein.,Dimattia MA, Watts NR, Stahl SJ, Grimes JM, Steven AC, Stuart DI, Wingfield PT Structure. 2013 Jan 8;21(1):133-42. doi: 10.1016/j.str.2012.10.017. Epub 2012 Dec, 6. PMID:23219881[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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