2o7l

From Proteopedia
Revision as of 13:57, 21 November 2007 by OCA (talk | contribs) (New page: left|200px<br /><applet load="2o7l" size="450" color="white" frame="true" align="right" spinBox="true" caption="2o7l, resolution 2.500Å" /> '''The open-cap confor...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigation Jump to search
File:2o7l.gif


2o7l, resolution 2.500Å

Drag the structure with the mouse to rotate

The open-cap conformation of GlpG

OverviewOverview

The active sites of intramembrane proteases are positioned in the lipid, bilayer to facilitate peptide bond hydrolysis in the membrane. Previous, crystallographic analysis of Escherichia coli GlpG, an intramembrane, protease of the rhomboid family, has revealed an internal and hydrophilic, active site in an apparently closed conformation. Here we describe the, crystal structure of GlpG in a more open conformation, where the capping, loop L5 has been lifted, exposing the previously buried and catalytically, essential Ser-201 to outside aqueous solution. A water molecule now moves, into the putative oxyanion hole that is constituted of a main-chain amide, (Ser-201) and two conserved side chains (His-150 and Asn-154). The loop, movement also destabilizes a hydrophobic side chain (Phe-245) previously, buried between transmembrane helices S2 and S5 and creates a side portal, from the lipid to protease active site. These results provide insights, into the conformational plasticity of GlpG to accommodate substrate, binding and catalysis and into the chirality of the reaction intermediate.

About this StructureAbout this Structure

2O7L is a Single protein structure of sequence from Escherichia coli with BNG as ligand. Full crystallographic information is available from OCA.

ReferenceReference

Open-cap conformation of intramembrane protease GlpG., Wang Y, Ha Y, Proc Natl Acad Sci U S A. 2007 Feb 13;104(7):2098-102. Epub 2007 Feb 2. PMID:17277078

Page seeded by OCA on Wed Nov 21 13:04:55 2007

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA