2hob
|
Crystal structure of SARS-CoV main protease with authentic N and C-termini in complex with a Michael acceptor N3
OverviewOverview
The viral proteases have proven to be the most selective and useful for, removing the fusion tags in fusion protein expression systems. As a key, enzyme in the viral life-cycle, the main protease (M(pro)) is most, attractive for drug design targeting the SARS coronavirus (SARS-CoV), the, etiological agent responsible for the outbreak of severe acute respiratory, syndrome (SARS) in 2003. In this study, SARS-CoV M(pro) was used to, specifically remove the GST tag in a new fusion protein expression system., We report a new method to produce wild-type (WT) SARS-CoV M(pro) with, authentic N and C termini, and compare the activity of WT protease with, those of three different types of SARS-CoV M(pro) with additional residues, at the N or C terminus. Our results show that additional residues at the N, terminus, but not at the C terminus, of M(pro) are detrimental to enzyme, activity. To explain this, the crystal structures of WT SARS-CoV M(pro), and its complex with a Michael acceptor inhibitor were determined to 1.6, Angstroms and 1.95 Angstroms resolution respectively. These crystal, structures reveal that the first residue of this protease is important for, sustaining the substrate-binding pocket and inhibitor binding. This study, suggests that SARS-CoV M(pro) could serve as a new tag-cleavage, endopeptidase for protein overproduction, and the WT SARS-CoV M(pro) is, more appropriate for mechanistic characterization and inhibitor design.
About this StructureAbout this Structure
2HOB is a Single protein structure of sequence from Sars coronavirus with 3IH as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Production of authentic SARS-CoV M(pro) with enhanced activity: application as a novel tag-cleavage endopeptidase for protein overproduction., Xue X, Yang H, Shen W, Zhao Q, Li J, Yang K, Chen C, Jin Y, Bartlam M, Rao Z, J Mol Biol. 2007 Feb 23;366(3):965-75. Epub 2006 Dec 1. PMID:17189639
Page seeded by OCA on Wed Nov 21 11:52:20 2007