1u0s
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Chemotaxis kinase CheA P2 domain in complex with response regulator CheY from the thermophile thermotoga maritima
OverviewOverview
Although interfaces mediating protein-protein interactions are thought to, be under strong evolutionary constraints, binding of the chemotaxis, histidine kinase CheA to its phosphorylation target CheY suggests, otherwise. The structure of Thermotoga maritima CheA domain P2 in complex, with CheY reveals a different association than that observed for the same, Escherichia coli proteins. Similar regions of CheY bind CheA P2 in the two, systems, but the CheA P2 domains differ by an approximately 90 degrees, rotation. CheA binds CheY with identical affinity in T. maritima and E., coli at the vastly different temperatures where the respective organisms, live. Distinct sets of P2 residues mediate CheY binding in the two, complexes; conservation patterns of these residues in CheA and, compensations in CheY delineate two families of prokaryotic chemotaxis, systems. A protein complex that has the same components and general, function in different organisms, but an altered structure, indicates, unanticipated complexity in the evolution of protein-protein interactions, and cautions against extrapolating structural data from homologs.
About this StructureAbout this Structure
1U0S is a Protein complex structure of sequences from Thermotoga maritima. Full crystallographic information is available from OCA.
ReferenceReference
In different organisms, the mode of interaction between two signaling proteins is not necessarily conserved., Park SY, Beel BD, Simon MI, Bilwes AM, Crane BR, Proc Natl Acad Sci U S A. 2004 Aug 10;101(32):11646-51. Epub 2004 Aug 2. PMID:15289606
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