1sw6
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S. CEREVISIAE SWI6 ANKYRIN-REPEAT FRAGMENT
OverviewOverview
Swi6 is a 92,000 Mr protein common to two distinct transcriptional, activation complexes (SBF and MBF) that coordinate gene expression at the, G1-S boundary of the yeast cell cycle. The X-ray structure of a central, 36,000 Mr fragment has been determined and refined at 2.1 A resolution., The structure reveals a basic framework of five ankyrin repeat modules, that is elaborated through a series of helical insertions distinguishing, it from structures of other ankyrin repeat proteins. A second domain, contains an approximately 30-residue region of extended structure that, interacts with the ankyrin repeat core over a substantial proportion of, its surface. Conservation of residues buried by these interactions, indicates that all members of the Swi6/Cdc10 family share a similar, architecture. Several temperature-sensitive mutations within Swi6 and, Cdc10 appear to disrupt these interdomain contacts rather than destabilize, the ankyrin repeat core. The unusual domain arrangement may be crucial for, the modulation of interactions with other co-regulatory molecules such as, cyclin-CDK complexes, and has implications for the quaternary interactions, within the multisubunit SBF and MBF transcription complexes.
About this StructureAbout this Structure
1SW6 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
X-ray structural analysis of the yeast cell cycle regulator Swi6 reveals variations of the ankyrin fold and has implications for Swi6 function., Foord R, Taylor IA, Sedgwick SG, Smerdon SJ, Nat Struct Biol. 1999 Feb;6(2):157-65. PMID:10048928
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