1nh2
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Crystal structure of a yeast TFIIA/TBP/DNA complex
OverviewOverview
RNA polymerase II-dependent transcription requires the assembly of a, multi-protein, preinitiation complex on core promoter elements., Transcription factor IID (TFIID) comprising the TATA box-binding protein, (TBP) and TBP-associated factors (TAFs) is responsible for promoter, recognition in this complex. Subsequent association of TFIIA and TFIIB, provides enhanced complex stability. TFIIA is required for transcriptional, stimulation by certain viral and cellular activators, and favors formation, of the preinitiation complex in the presence of repressor NC2. The X-ray, structures of human and yeast TBP/TFIIA/DNA complexes at 2.1A and 1.9A, resolution, respectively, are presented here and seen to resemble each, other closely. The interactions made by human TFIIA with TBP and DNA, within and upstream of the TATA box, including those involving water, molecules, are described and compared to the yeast structure. Of, particular interest is a previously unobserved region of TFIIA that, extends the binding interface with TBP in the yeast, but not in the human, complex, and that further elucidates biochemical and genetic results.
About this StructureAbout this Structure
1NH2 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
ReferenceReference
Novel interactions between the components of human and yeast TFIIA/TBP/DNA complexes., Bleichenbacher M, Tan S, Richmond TJ, J Mol Biol. 2003 Sep 26;332(4):783-93. PMID:12972251
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