1k8g
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Crystal Structure of the N-terminal domain of Oxytricha nova telomere end binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA
OverviewOverview
Oxytricha nova telomere end-binding protein specifically recognizes and, caps single strand (T(4)G(4))(n) telomeric DNA at the very 3'-ends of O., nova macronuclear chromosomes. Proteins homologous to the N-terminal, domain of OnTEBP alpha subunit have now been identified in Oxytricha, trifallax, Stylonychia mytilis, Euplotes crassus, Schizosaccharomyces, pombe, and Homo sapiens, suggesting that this protein is widely, distributed in eukaryotes. We describe here the crystal structures of the, N-terminal single-stranded DNA (ssDNA)-binding domain of O. nova telomere, end-binding protein alpha subunit both uncomplexed and complexed with, single strand telomeric DNA. These structures show how the N-terminal, domain of alpha alone, in the absence of the beta subunit and without, alpha dimerization, can bind single-stranded telomeric DNA in a, sequence-specific and 3'-end-specific manner. Furthermore, comparison of, the uncomplexed and complexed forms of this protein shows that the, ssDNA-binding site is largely pre-organized in the absence of ssDNA with, modest, but interesting, rearrangements of amino acid side-chains that, compose the ssDNA-binding site. The structures described here extend our, understanding of structures of O. nova telomeric complexes by adding, uncomplexed and complexed forms of monomeric alpha to previously described, structures for (alpha 56/ssDNA)(2) dimer and alpha 56/beta 28/ssDNA, ternary complexes. We believe that each of these four structures represent, intermediates in an ordered assembly/disassembly pathway for O. nova, telomeric complexes.
About this StructureAbout this Structure
1K8G is a Single protein structure of sequence from Sterkiella nova with SO4 as ligand. Full crystallographic information is available from OCA.
ReferenceReference
Crystal structure of the N-terminal domain of Oxytricha nova telomere end-binding protein alpha subunit both uncomplexed and complexed with telomeric ssDNA., Classen S, Ruggles JA, Schultz SC, J Mol Biol. 2001 Dec 14;314(5):1113-25. PMID:11743727
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