1hht
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RNA DEPENDENT RNA POLYMERASE FROM DSRNA BACTERIOPHAGE PHI6 PLUS TEMPLATE
OverviewOverview
In most RNA viruses, genome replication and transcription are catalysed by, a viral RNA-dependent RNA polymerase. Double-stranded RNA viruses perform, these operations in a capsid (the polymerase complex), using an enzyme, that can read both single- and double-stranded RNA. Structures have been, solved for such viral capsids, but they do not resolve the polymerase, subunits in any detail. Here we show that the 2 A resolution X-ray, structure of the active polymerase subunit from the double-stranded RNA, bacteriophage straight phi6 is highly similar to that of the polymerase of, hepatitis C virus, providing an evolutionary link between double-stranded, RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we determined a number of other structures, including complexes ... [(full description)]
About this StructureAbout this Structure
1HHT is a [Single protein] structure of sequence from [Bacteriophage phi-6] with MN as [ligand]. Full crystallographic information is available from [OCA].
ReferenceReference
A mechanism for initiating RNA-dependent RNA polymerization., Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI, Nature. 2001 Mar 8;410(6825):235-40. PMID:11242087
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