1fi8
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| , resolution 2.2Å | |||||||
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
RAT GRANZYME B [N66Q] COMPLEXED TO ECOTIN [81-84 IEPD]
OverviewOverview
Granzyme B is a serine protease of the chymotrypsin fold that mediates cell death by cytotoxic lymphocytes. It is a processing enzyme, requiring extended peptide substrates containing an Asp residue. The determinants that allow for this substrate specificity are revealed in the three-dimensional structure of granzyme B in complex with a macromolecular inhibitor. The primary specificity for Asp occurs through a side-on interaction with Arg 226, a buried Arg side chain of granzyme B. An additional nine amino acids make contact with the substrate and define the granzyme B extended substrate specificity profile. The substrate determinants found in this structure are shared by other members of this protein class and help to reveal the properties that define substrate specificity.
About this StructureAbout this Structure
1FI8 is a Protein complex structure of sequences from Escherichia coli and Rattus norvegicus. Full crystallographic information is available from OCA.
ReferenceReference
The structure of the pro-apoptotic protease granzyme B reveals the molecular determinants of its specificity., Waugh SM, Harris JL, Fletterick R, Craik CS, Nat Struct Biol. 2000 Sep;7(9):762-5. PMID:10966646
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