CCA-adding enzyme

<StructureSection load='3ovs' size='350' side='right' caption='Structure of CCA-adding enzyme complex with tRNA 34-mer, CTP, sulfate, ethanediol and Ca+2 ion (PDB entry 3ovs)' scene=>

CCA-adding enzyme (CAE) builds or repairs the trinucleotice CCA at the 3’ end of tRNA. CAE has one active site which is capable of binding CTP and ATP in a specific sequence. In the bacteria Aquifex aeolicus, CAE consists of 2 subunits. One specific for CTP and the other for ATP. CAE builds the CCA without a genetic template.

3D structures of CCA-adding enzyme3D structures of CCA-adding enzyme

Updated on 20-June-2013

1miv – BsCAE – Bacillus stearothermophilus
1uet, 1r8a – AfCAE – Archaeoglobus fulgidus

CCA-adding enzyme binary complex with nucleotideCCA-adding enzyme binary complex with nucleotide

1miw – BsCAE + ATP
1uev, 1r8b – AfCAE + ATP
1miy – BsCAE + CTP
1ueu, 1r89 – AfCAE + CTP
1r8c – AfCAE + UTP

CCA-adding enzyme binary complex with tRNACCA-adding enzyme binary complex with tRNA

2dr5 – AfCAE + tRNA 32-mer
2dr7, 2zh1, 2zh7, 2zh9 – AfCAE + tRNA 33-mer
2dr9, 2zh2, 2zh3, 2zh4, 2zh5, 2zh8, 2zhb – AfCAE + tRNA 34-mer
2drb, 3ouy – AfCAE + tRNA 35-mer

CCA-adding enzyme ternary complex with nucleotide and tRNACCA-adding enzyme ternary complex with nucleotide and tRNA

1sz1 – AfCAE + GTP + UMP + CMP + tRNA
1tfw – AfCAE + ATP + tRNA 3’ end
1tfy – AfCAE + CTP + tRNA 3’ end
2dr8, 2zha – AfCAE + CTP + tRNA 33-mer
2dra, 2zh6, 3ov7 – AfCAE + ATP + tRNA 34-mer
3ova – AfCAE + ATP derivative + tRNA 34-mer
2dvi, 3ovs – AfCAE + CTP + tRNA 34-mer
3ovb – AfCAE + ATP + tRNA 35-mer