2cdg

File:2cdg.gif

spinqualitylabelsall models 2cdg, resolution 2.60Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

STRUCTURE AND BINDING KINETICS OF THREE DIFFERENT HUMAN CD1D-ALPHA-GALACTOSYLCERAMIDE-SPECIFIC T CELL RECEPTORS (TCR 5B)

OverviewOverview

Invariant human TCR Valpha24-Jalpha18+/Vbeta11+ NKT cells (iNKT) are, restricted by CD1d-alpha-glycosylceramides. We analyzed crystal structures, and binding characteristics for an iNKT TCR plus two, CD1d-alpha-GalCer-specific Vbeta11+ TCRs that use different TCR Valpha, chains. The results were similar to those previously reported for, MHC-peptide-specific TCRs, illustrating the versatility of the TCR, platform. Docking TCR and CD1d-alpha-GalCer structures provided plausible, insights into their interaction. The model supports a diagonal orientation, of TCR on CD1d and suggests that complementarity determining region, (CDR)3alpha, CDR3beta, and CDR1beta interact with ligands presented by, CD1d, whereas CDR2beta binds to the CD1d alpha1 helix. This docking, provides an explanation for the dominant usage of Vbeta11 and Vbeta8.2, chains by human and mouse iNKT cells, respectively, for recognition of, CD1d-alpha-GalCer.

About this StructureAbout this Structure

2CDG is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

ReferenceReference

Structure and binding kinetics of three different human CD1d-alpha-galactosylceramide-specific T cell receptors., Gadola SD, Koch M, Marles-Wright J, Lissin NM, Shepherd D, Matulis G, Harlos K, Villiger PM, Stuart DI, Jakobsen BK, Cerundolo V, Jones EY, J Exp Med. 2006 Mar 20;203(3):699-710. Epub 2006 Mar 6. PMID:16520393

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