7oxr

From Proteopedia
Revision as of 23:17, 20 October 2021 by OCA (talk | contribs)
Jump to navigation Jump to search

Cryo-EM structure of yeast Sei1 with locking helix deletionCryo-EM structure of yeast Sei1 with locking helix deletion

Structural highlights

7oxr is a 10 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Publication Abstract from PubMed

Lipid droplets (LDs) are universal lipid storage organelles with a core of neutral lipids, such as triacylglycerols, surrounded by a phospholipid monolayer. This unique architecture is generated during LD biogenesis at endoplasmic reticulum (ER) sites marked by Seipin, a conserved membrane protein mutated in lipodystrophy. Here structural, biochemical and molecular dynamics simulation approaches reveal the mechanism of LD formation by the yeast Seipin Sei1 and its membrane partner Ldb16. We show that Sei1 luminal domain assembles a homooligomeric ring, which, in contrast to other Seipins, is unable to concentrate triacylglycerol. Instead, Sei1 positions Ldb16, which concentrates triacylglycerol within the Sei1 ring through critical hydroxyl residues. Triacylglycerol recruitment to the complex is further promoted by Sei1 transmembrane segments, which also control Ldb16 stability. Thus, we propose that LD assembly by the Sei1/Ldb16 complex, and likely other Seipins, requires sequential triacylglycerol-concentrating steps via distinct elements in the ER membrane and lumen.

Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex.,Klug YA, Deme JC, Corey RA, Renne MF, Stansfeld PJ, Lea SM, Carvalho P Nat Commun. 2021 Oct 8;12(1):5892. doi: 10.1038/s41467-021-26162-6. PMID:34625558[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

  1. Klug YA, Deme JC, Corey RA, Renne MF, Stansfeld PJ, Lea SM, Carvalho P. Mechanism of lipid droplet formation by the yeast Sei1/Ldb16 Seipin complex. Nat Commun. 2021 Oct 8;12(1):5892. doi: 10.1038/s41467-021-26162-6. PMID:34625558 doi:http://dx.doi.org/10.1038/s41467-021-26162-6

7oxr, resolution 3.30Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA