Crystal structure of tRNA-intron endonuclease from Sulfolobus tokodaiiCrystal structure of tRNA-intron endonuclease from Sulfolobus tokodaii

Structural highlights

2cv8 is a 2 chain structure with sequence from Dsm 16993. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
NonStd Res:
Activity:tRNA-intron endonuclease, with EC number 3.1.27.9
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[ENDA_SULTO] Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp (By similarity).

Evolutionary Conservation

 

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2cv8, resolution 2.80Å

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