2m3c

Solution Structure of gammaM7-CrystallinSolution Structure of gammaM7-Crystallin

Structural highlights 2m3c is a 1 chain structure with sequence from Danio rerio. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance. Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function Q5XTN3_DANRE Publication Abstract from PubMed The vertebrate eye lens contains high concentrations of crystallins. The dense lenses of fish are particularly abundant in a class called gammaM-crystallin whose members are characterized by an unusually high methionine content and partial loss of the four tryptophan residues conserved in all gamma-crystallins from mammals which are proposed to contribute to protection from UV-damage. Here, we present the structure and dynamics of gammaM7-crystallin from zebrafish (Danio rerio). The solution structure shares the typical two-domain, four-Greek-key motif arrangement of other gamma-crystallins, with the major difference noted in the final loop of the N-terminal domain, spanning residues 65-72. This is likely due to the absence of the conserved tryptophans. Many of the methionine residues are exposed on the surface but are mostly well-ordered and frequently have contacts with aromatic side chains. This may contribute to the specialized surface properties of these proteins that exist under high molecular crowding in the fish lens. NMR relaxation data show increased backbone conformational motions in the loop regions of gammaM7 compared to those of mouse gammaS-crystallin and show that fast internal motion of the interdomain linker in gamma-crystallins correlates with linker length. Unfolding studies monitored by tryptophan fluorescence confirm results from mutant mouse gammaS-crystallin and show that unfolding of a betagamma-crystallin domain likely starts from unfolding of the variable loop containing the more fluorescently quenched tryptophan residue, resulting in a native-like unfolding intermediate. Structure and Dynamics of the Fish Eye Lens Protein, gammaM7-Crystallin.,Mahler B, Chen Y, Ford J, Thiel C, Wistow G, Wu Z Biochemistry. 2013 May 8. PMID:23597261 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Crystallin 3D structures

spinqualitylabelsall models 2m3c Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image