2xkv

Atomic Model of the SRP-FtsY Early ConformationAtomic Model of the SRP-FtsY Early Conformation

2xkv, resolution 13.50Å Show:Asymmetric Unit Biological Assembly Structural highlights 2xkv is a 4 chain structure with sequence from Escherichia coli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 13.5Å Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function SRP54_THEAQ Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY (By similarity).[HAMAP-Rule:MF_00306] Publication Abstract from PubMed We report the 'early' conformation of the Escherichia coli signal recognition particle (SRP) and its receptor FtsY bound to the translating ribosome, as determined by cryo-EM. FtsY binds to the tetraloop of the SRP RNA, whereas the NG domains of the SRP protein and FtsY interact weakly in this conformation. Our results suggest that optimal positioning of the SRP RNA tetraloop and the Ffh NG domain leads to FtsY recruitment. Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor.,Estrozi LF, Boehringer D, Shan SO, Ban N, Schaffitzel C Nat Struct Mol Biol. 2011 Jan;18(1):88-90. Epub 2010 Dec 12. PMID:21151118 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Signal recognition particle protein Signal recognition particle receptor