2kro
RDC refined high resolution structure of the third SH3 domain of CD2APRDC refined high resolution structure of the third SH3 domain of CD2AP
Structural highlights
FunctionCD2AP_MOUSE Required for cytokinesis (By similarity). Seems to act as an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus.[1] Publication Abstract from PubMedCD2 associated protein (CD2AP) is an adaptor protein that plays an important role in cell to cell union needed for the kidney function. It contains three N-terminal SH3 domains that are able to interact among others with CD2, ALIX, c-Cbl and Ubiquitin. To understand the role of the individual SH3 domains of this adaptor protein we have performed a complete structural, thermodynamic and dynamic characterization of the separate domains using NMR and DSC. The energetic contributions to the stability and the backbone dynamics have been related to the structural features of each domain using the structure-based FoldX algorithm. We have found that the N-terminal SH3 domain of both adaptor proteins CD2AP and CIN85 are the most stable SH3 domains that have been studied until now. This high stability is driven by a more extensive network of intra-molecular interactions. We believe that this increased stabilization of N-terminal SH3 domains in adaptor proteins is crucial to maintain the necessary conformation to establish the proper interactions critical for the recruitment of their natural targets. Solution structure, dynamics and thermodynamics of the three SH3 domains of CD2AP.,Roldan JL, Blackledge M, van Nuland NA, Azuaga AI J Biomol NMR. 2011 Jun;50(2):103-17. doi: 10.1007/s10858-011-9505-5. Epub 2011 , Apr 26. PMID:21519904[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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