5zfu

Structure of the ExbB/ExbD hexameric complex (ExbB6ExbD3TM)Structure of the ExbB/ExbD hexameric complex (ExbB6ExbD3TM)

5zfu, resolution 6.70Å Show:Asymmetric Unit Biological Assembly Structural highlights 5zfu is a 9 chain structure with sequence from Ecoli. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Gene: exbB, b3006, JW2974 (ECOLI), exbD, b3005, JW2973 (ECOLI) Experimental data: Check Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [EXBB_ECOLI] Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Protects ExbD from proteolytic degradation and functionally stabilizes TonB. [EXBD_ECOLI] Involved in the TonB-dependent energy-dependent transport of various receptor-bound substrates. Publication Abstract from PubMed Gram-negative bacteria import essential nutrients such as iron and vitamin B12 through outer membrane receptors. This process utilizes proton motive force harvested by the Ton system made up of three inner membrane proteins, ExbB, ExbD and TonB. ExbB and ExbD form the proton channel that energizes uptake through TonB. Recently, crystal structures suggest that the ExbB pentamer is the scaffold. Here, we present structures of hexameric complexes of ExbB and ExbD revealed by X-ray crystallography and single particle cryo-EM. Image analysis shows that hexameric and pentameric complexes coexist, with the proportion of hexamer increasing with pH. Channel current measurement and 2D crystallography support the existence and transition of the two oligomeric states in membranes. The hexameric complex consists of six ExbB subunits and three ExbD transmembrane helices enclosed within the central channel. We propose models for activation/inactivation associated with hexamer and pentamer formation and utilization of proton motive force. Hexameric and pentameric complexes of the ExbBD energizer in the Ton system.,Maki-Yonekura S, Matsuoka R, Yamashita Y, Shimizu H, Tanaka M, Iwabuki F, Yonekura K Elife. 2018 Apr 17;7. pii: 35419. doi: 10.7554/eLife.35419. PMID:29661272 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also ExbB