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Structure of Rpf2-Rrs1 complex involved in ribosome biogenesisStructure of Rpf2-Rrs1 complex involved in ribosome biogenesis
Structural highlights
FunctionPublication Abstract from PubMedProteins Rpf2 and Rrs1 are required for 60S ribosomal subunit maturation. These proteins are necessary for the recruitment of three ribosomal components (5S ribosomal RNA [rRNA], RpL5 and RpL11) to the 90S ribosome precursor and subsequent 27SB pre-rRNA processing. Here we present the crystal structure of the Aspergillus nidulans (An) Rpf2-Rrs1 core complex. The core complex contains the tightly interlocked N-terminal domains of Rpf2 and Rrs1. The Rpf2 N-terminal domain includes a Brix domain characterized by similar N- and C-terminal architecture. The long alpha-helix of Rrs1 joins the C-terminal half of the Brix domain as if it were part of a single molecule. The conserved proline-rich linker connecting the N- and C-terminal domains of Rrs1 wrap around the side of Rpf2 and anchor the C-terminal domain of Rrs1 to a specific site on Rpf2. In addition, gel shift analysis revealed that the Rpf2-Rrs1 complex binds directly to 5S rRNA. Further analysis of Rpf2-Rrs1 mutants demonstrated that Saccharomyces cerevisiae Rpf2 R236 (corresponds to R238 of AnRpf2) plays a significant role in this binding. Based on these studies and previous reports, we have proposed a model for ribosomal component recruitment to the 90S ribosome precursor. Structural and functional analysis of the Rpf2-Rrs1 complex in ribosome biogenesis.,Asano N, Kato K, Nakamura A, Komoda K, Tanaka I, Yao M Nucleic Acids Res. 2015 May 19;43(9):4746-57. doi: 10.1093/nar/gkv305. Epub 2015 , Apr 8. PMID:25855814[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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