3kx2

Crystal structure of Prp43p in complex with ADPCrystal structure of Prp43p in complex with ADP

Structural highlights

3kx2 is a 2 chain structure with sequence from Baker's yeast. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	YGL120C (Baker's yeast)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PRP43_YEAST] Pre-mRNA processing factor involved in disassembly of spliceosomes after the release of mature mRNA.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DEAH helicases participate in pre-messenger RNA splicing and ribosome biogenesis. The structure of yeast Prp43p-ADP reveals the homology of DEAH helicases to DNA helicases and the presence of an oligonucleotide-binding motif. A beta-hairpin from the second RecA domain is wedged between two carboxy-terminal domains and blocks access to the occluded RNA binding site formed by the RecA domains and a C-terminal domain. ATP binding and hydrolysis are likely to induce conformational changes in the hairpin that are important for RNA unwinding or ribonucleoprotein remodelling. The structure of Prp43p provides the framework for functional and genetic analysis of all DEAH helicases.

Structural basis for the function of DEAH helicases.,He Y, Andersen GR, Nielsen KH EMBO Rep. 2010 Mar;11(3):180-6. Epub 2010 Feb 19. PMID:20168331^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Arenas JE, Abelson JN. Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11798-802. PMID:9342317
↑ Boon KL, Auchynnikava T, Edwalds-Gilbert G, Barrass JD, Droop AP, Dez C, Beggs JD. Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol Cell Biol. 2006 Aug;26(16):6016-23. PMID:16880513 doi:http://dx.doi.org/10.1128/MCB.02347-05
↑ He Y, Andersen GR, Nielsen KH. Structural basis for the function of DEAH helicases. EMBO Rep. 2010 Mar;11(3):180-6. Epub 2010 Feb 19. PMID:20168331 doi:10.1038/embor.2010.11

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OCA

[1] Arenas JE, Abelson JN. Prp43: An RNA helicase-like factor involved in spliceosome disassembly. Proc Natl Acad Sci U S A. 1997 Oct 28;94(22):11798-802. PMID:9342317

[2] Boon KL, Auchynnikava T, Edwalds-Gilbert G, Barrass JD, Droop AP, Dez C, Beggs JD. Yeast ntr1/spp382 mediates prp43 function in postspliceosomes. Mol Cell Biol. 2006 Aug;26(16):6016-23. PMID:16880513 doi:http://dx.doi.org/10.1128/MCB.02347-05

[3] He Y, Andersen GR, Nielsen KH. Structural basis for the function of DEAH helicases. EMBO Rep. 2010 Mar;11(3):180-6. Epub 2010 Feb 19. PMID:20168331 doi:10.1038/embor.2010.11

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