Structural highlights3ui2 is a 2 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Related: | |
| Gene: | At2g47450, CAO, CPSRP43, T30B22.25 (ARATH), At5g03940, CPSRP54, F8F6_150, FFC (ARATH) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function[SR43C_ARATH] Component of the chloroplast signal recognition particle pathway. Required for post-translational targeting of proteins into the tylakoid membrane but seems to be dispensable for co-translational targeting with a translating ribosome present. May be able to function independently of cpFTSY and FFC/cpSRP54 in targeting LHCPs to the thylakoids. Acts as a highly specific chaperone for LHCPs, preventing aggregation and being able to dissolve aggregates.[1] [2] [3] [4] [5] [SR54C_ARATH] Involved in cotranslational and post-translational sorting of thylakoid proteins. Binds GTP specifically. Activates the GTPase activity of CPFTSY when bound together. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids.[6] [7] [8] [9]
Publication Abstract from PubMed
Chromodomains typically recruit protein complexes to chromatin and read the epigenetic histone code by recognizing lysine methylation in histone tails. We report the crystal structure of the chloroplast signal recognition particle (cpSRP) core from Arabidopsis thaliana, with the cpSRP54 tail comprising an arginine-rich motif bound to the second chromodomain of cpSRP43. A twinned aromatic cage reads out two neighboring nonmethylated arginines and adapts chromodomains to a non-nuclear function in post-translational targeting.
Chromodomains read the arginine code of post-translational targeting.,Holdermann I, Meyer NH, Round A, Wild K, Sattler M, Sinning I Nat Struct Mol Biol. 2012 Jan 8;19(2):260-3. doi: 10.1038/nsmb.2196. PMID:22231402[10]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See AlsoReferences
- ↑ Klimyuk VI, Persello-Cartieaux F, Havaux M, Contard-David P, Schuenemann D, Meiherhoff K, Gouet P, Jones JD, Hoffman NE, Nussaume L. A chromodomain protein encoded by the arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting. Plant Cell. 1999 Jan;11(1):87-99. PMID:9878634
- ↑ Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999 Sep 17;274(38):27219-24. PMID:10480939
- ↑ Goforth RL, Peterson EC, Yuan J, Moore MJ, Kight AD, Lohse MB, Sakon J, Henry RL. Regulation of the GTPase cycle in post-translational signal recognition particle-based protein targeting involves cpSRP43. J Biol Chem. 2004 Oct 8;279(41):43077-84. Epub 2004 Aug 2. PMID:15292240 doi:http://dx.doi.org/10.1074/jbc.M401600200
- ↑ Tzvetkova-Chevolleau T, Hutin C, Noel LD, Goforth R, Carde JP, Caffarri S, Sinning I, Groves M, Teulon JM, Hoffman NE, Henry R, Havaux M, Nussaume L. Canonical signal recognition particle components can be bypassed for posttranslational protein targeting in chloroplasts. Plant Cell. 2007 May;19(5):1635-48. Epub 2007 May 18. PMID:17513500 doi:http://dx.doi.org/10.1105/tpc.106.048959
- ↑ Falk S, Sinning I. cpSRP43 is a novel chaperone specific for light-harvesting chlorophyll a,b-binding proteins. J Biol Chem. 2010 Jul 9;285(28):21655-61. doi: 10.1074/jbc.C110.132746. Epub 2010, May 24. PMID:20498370 doi:http://dx.doi.org/10.1074/jbc.C110.132746
- ↑ Tu CJ, Schuenemann D, Hoffman NE. Chloroplast FtsY, chloroplast signal recognition particle, and GTP are required to reconstitute the soluble phase of light-harvesting chlorophyll protein transport into thylakoid membranes. J Biol Chem. 1999 Sep 17;274(38):27219-24. PMID:10480939
- ↑ Groves MR, Mant A, Kuhn A, Koch J, Dubel S, Robinson C, Sinning I. Functional characterization of recombinant chloroplast signal recognition particle. J Biol Chem. 2001 Jul 27;276(30):27778-86. Epub 2001 May 16. PMID:11356852 doi:http://dx.doi.org/10.1074/jbc.M103470200
- ↑ Jaru-Ampornpan P, Chandrasekar S, Shan SO. Efficient interaction between two GTPases allows the chloroplast SRP pathway to bypass the requirement for an SRP RNA. Mol Biol Cell. 2007 Jul;18(7):2636-45. Epub 2007 May 2. PMID:17475780 doi:http://dx.doi.org/10.1091/mbc.E07-01-0037
- ↑ Rutschow H, Ytterberg AJ, Friso G, Nilsson R, van Wijk KJ. Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis. Plant Physiol. 2008 Sep;148(1):156-75. doi: 10.1104/pp.108.124545. Epub 2008 Jul , 16. PMID:18633119 doi:http://dx.doi.org/10.1104/pp.108.124545
- ↑ Holdermann I, Meyer NH, Round A, Wild K, Sattler M, Sinning I. Chromodomains read the arginine code of post-translational targeting. Nat Struct Mol Biol. 2012 Jan 8;19(2):260-3. doi: 10.1038/nsmb.2196. PMID:22231402 doi:10.1038/nsmb.2196
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