3snp

Crystal structure analysis of iron regulatory protein 1 in complex with ferritin H IRE RNACrystal structure analysis of iron regulatory protein 1 in complex with ferritin H IRE RNA

Structural highlights 3snp is a 4 chain structure with sequence from European rabbit. This structure supersedes the now removed PDB entry 2ipy. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Related: 2ipy, 3sn2 Gene: ACO1, FRP, IREB1, IREBP, IRP1 (European rabbit) Activity: Aconitate hydratase, with EC number 4.2.1.3 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [ACOC_RABIT] Iron sensor. Binds a 4Fe-4S cluster and functions as aconitase when cellular iron levels are high. Functions as mRNA binding protein that regulates uptake, sequestration and utilization of iron when cellular iron levels are low. Binds to iron-responsive elements (IRES) in target mRNA species when iron levels are low. Binding of a 4Fe-4S cluster precludes RNA binding (By similarity). Catalyzes the isomerization of citrate to isocitrate via cis-aconitate (By similarity). Publication Abstract from PubMed Iron regulatory protein 1 (IRP1) binds iron-responsive elements (IREs) in messenger RNAs (mRNAs), to repress translation or degradation, or binds an iron-sulfur cluster, to become a cytosolic aconitase enzyme. The 2.8 angstrom resolution crystal structure of the IRP1:ferritin H IRE complex shows an open protein conformation compared with that of cytosolic aconitase. The extended, L-shaped IRP1 molecule embraces the IRE stem-loop through interactions at two sites separated by approximately 30 angstroms, each involving about a dozen protein:RNA bonds. Extensive conformational changes related to binding the IRE or an iron-sulfur cluster explain the alternate functions of IRP1 as an mRNA regulator or enzyme. Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA.,Walden WE, Selezneva AI, Dupuy J, Volbeda A, Fontecilla-Camps JC, Theil EC, Volz K Science. 2006 Dec 22;314(5807):1903-8. PMID:17185597 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See Also Aconitase 3D structures

spinqualitylabelsall models 3snp, resolution 2.80Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image