3px7

Crystal Structure of covalent complex of topoisomerase 1A with substrateCrystal Structure of covalent complex of topoisomerase 1A with substrate

Structural highlights 3px7 is a 3 chain structure with sequence from Ecod1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: NonStd Res: Related: 1ecl, 1cy0, 1cy4 Gene: 415337, EcDH1_2375 (ECOD1) Activity: DNA topoisomerase, with EC number 5.99.1.2 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Function [C9QXS7_ECOD1] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952][SAAS:SAAS00003469] See Also Topoisomerase 3D structures

spinqualitylabelsall models 3px7, resolution 2.30Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image