3ub7

Periplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide boundPeriplasmic portion of the Helicobacter pylori chemoreceptor TlpB with acetamide bound

Structural highlights 3ub7 is a 2 chain structure with sequence from Helicobacter pylori (strain ss1). The February 2013 RCSB PDB Molecule of the Month feature on Proton-Gated Urea Channel by David Goodsell is 10.2210/rcsb_pdb/mom_2013_2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Ligands: , , Related: 3ub6, 3ub8, 3ub9 Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed pH sensing is crucial for survival of most organisms, yet the molecular basis of such sensing is poorly understood. Here, we present an atomic resolution structure of the periplasmic portion of the acid-sensing chemoreceptor, TlpB, from the gastric pathogen Helicobacter pylori. The structure reveals a universal signaling fold, a PAS domain, with a molecule of urea bound with high affinity. Through biophysical, biochemical, and in vivo mutagenesis studies, we show that urea and the urea-binding site residues play critical roles in the ability of H. pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding. Structure and Proposed Mechanism for the pH-Sensing Helicobacter pylori Chemoreceptor TlpB.,Goers Sweeney E, Henderson JN, Goers J, Wreden C, Hicks KG, Foster JK, Parthasarathy R, Remington SJ, Guillemin K Structure. 2012 Jun 14. PMID:22705207 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

spinqualitylabelsall models 3ub7, resolution 1.40Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image