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The structure of protozoan parasite Trichomonas vaginalis Myb2 in complex with MRE-2f-13 DNAThe structure of protozoan parasite Trichomonas vaginalis Myb2 in complex with MRE-2f-13 DNA
Structural highlights
Publication Abstract from PubMedIron-inducible transcription of the ap65-1 gene in Trichomonas vaginalis involves at least three Myb-like transcriptional factors (tvMyb1, tvMyb2 and tvMyb3) that differentially bind to two closely spaced promoter sites, MRE-1/MRE-2r and MRE-2f. Here, we defined a fragment of tvMyb2 comprising residues 40-156 (tvMyb2(40-156)) as the minimum structural unit that retains near full binding affinity with the promoter DNAs. Like c-Myb in vertebrates, the DNA-free tvMyb2(40-156) has a flexible and open conformation. Upon binding to the promoter DNA elements, tvMyb2(40-156) undergoes significant conformational re-arrangement and structure stabilization. Crystal structures of tvMyb2(40-156) in complex with promoter element-containing DNA oligomers showed that 5'-a/gACGAT-3' is the specific base sequence recognized by tvMyb2(40-156), which does not fully conform to that of the Myb binding site sequence. Furthermore, Lys(49), which is upstream of the R2 motif (amino acids 52-102) also participates in specific DNA sequence recognition. Intriguingly, tvMyb2(40-156) binds to the promoter elements in an orientation opposite to that proposed in the HADDOCK model of the tvMyb1(35-141)/MRE-1-MRE-2r complex. These results shed new light on understanding the molecular mechanism of Myb-DNA recognition and provide a framework to study the molecular basis of transcriptional regulation of myriad Mybs in T. vaginalis. Molecular basis of the recognition of the ap65-1 gene transcription promoter elements by a Myb protein from the protozoan parasite Trichomonas vaginalis.,Jiang I, Tsai CK, Chen SC, Wang SH, Amiraslanov I, Chang CF, Wu WJ, Tai JH, Liaw YC, Huang TH Nucleic Acids Res. 2011 Jul 19. PMID:21771861[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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