2x0h

BtGH84 Michaelis complexBtGH84 Michaelis complex

Structural highlights

2x0h is a 2 chain structure with sequence from Bactn. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	2vvn, 2wzh, 2w67, 2wca, 2vvs, 2j4g, 2w4x, 2cho, 2jiw, 2chn, 2j47, 2wzi, 2w66
Activity:	Beta-N-acetylhexosaminidase, with EC number 3.2.1.52
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[OGA_BACTN] Biological function unknown. Capable of hydrolyzing the glycosidic link of O-GlcNAcylated proteins.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

N-Acetylglucosamine beta-O-linked to serine and threonine residues of nucleocytoplasmic proteins (O-GlcNAc) has been linked to neurodegeneration, cellular stress response, and transcriptional regulation. Removal of O-GlcNAc is catalyzed by O-GlcNAcase (OGA) using a substrate-assisted catalytic mechanism. Here we define the reaction coordinate using chemical approaches and directly observe both a Michaelis complex and the oxazoline intermediate.

Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase.,He Y, Macauley MS, Stubbs KA, Vocadlo DJ, Davies GJ J Am Chem Soc. 2010 Jan 12. PMID:20067256^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ He Y, Macauley MS, Stubbs KA, Vocadlo DJ, Davies GJ. Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase. J Am Chem Soc. 2010 Jan 12. PMID:20067256 doi:10.1021/ja9086769

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OCA

[1] He Y, Macauley MS, Stubbs KA, Vocadlo DJ, Davies GJ. Visualizing the Reaction Coordinate of an O-GlcNAc Hydrolase. J Am Chem Soc. 2010 Jan 12. PMID:20067256 doi:10.1021/ja9086769

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