3uw6
Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR120Crystal Structure of Engineered Protein, Northeast Structural Genomics Consortium Target OR120
Structural highlights
Publication Abstract from PubMedThe Morita-Baylis-Hillman reaction forms a carbon-carbon bond between the alpha-carbon of a conjugated carbonyl compound and a carbon electrophile. The reaction mechanism involves Michael addition of a nucleophile catalyst at the carbonyl beta-carbon, followed by bond formation with the electrophile and catalyst disassociation to release the product. We used Rosetta to design 48 proteins containing active sites predicted to carry out this mechanism, of which two show catalytic activity by mass spectrometry (MS). Substrate labeling measured by MS and site-directed mutagenesis experiments show that the designed active-site residues are responsible for activity, although rate acceleration over background is modest. To characterize the designed proteins, we developed a fluorescence-based screen for intermediate formation in cell lysates, carried out microsecond molecular dynamics simulations, and solved X-ray crystal structures. These data indicate a partially formed active site and suggest several clear avenues for designing more active catalysts. Computational design of enone-binding proteins with catalytic activity for the Morita-Baylis-Hillman reaction.,Bjelic S, Nivon LG, Celebi-Olcum N, Kiss G, Rosewall CF, Lovick HM, Ingalls EL, Gallaher JL, Seetharaman J, Lew S, Montelione GT, Hunt JF, Michael FE, Houk KN, Baker D ACS Chem Biol. 2013 Apr 19;8(4):749-57. doi: 10.1021/cb3006227. Epub 2013 Jan 30. PMID:23330600[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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