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TANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURESTANDEM GU MISMATCHES IN RNA, NMR, 30 STRUCTURES
Structural highlights
Publication Abstract from PubMedThe symmetric, tandem GU mismatch motifs, and , which only differ in the mismatch order, have an average difference in thermodynamic stability of 2 kcal/mol at 37 degrees C. Thermodynamic studies of duplexes containing these motifs indicate the effect is largely localized to the mismatches and adjacent base pairs. The three-dimensional structures of two representative duplexes, (rGGAGUUCC)2 and (rGGAUGUCC)2, were determined by two-dimensional NMR and a simulated annealing protocol. Local deviations are similar to other intrahelical GU mismatches with little effect on backbone torsion angles and a slight overtwisting between the base pair 5' of the G of the mismatch and the mismatch itself. Comparisons of the resulting stacking patterns along with electrostatic potential maps suggest that interactions between highly negative electrostatic regions between base pairs may play a role in the observed thermodynamic differences. Investigation of the structural basis for thermodynamic stabilities of tandem GU wobble pairs: NMR structures of (rGGAGUUCC)2 and (rGGAUGUCC)2.,McDowell JA, He L, Chen X, Turner DH Biochemistry. 1997 Jul 1;36(26):8030-8. PMID:9201950[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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