3nmv

From Proteopedia
Revision as of 11:33, 8 November 2017 by OCA (talk | contribs)
Jump to navigation Jump to search

Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2Crystal structure of pyrabactin-bound abscisic acid receptor PYL2 mutant A93F in complex with type 2C protein phosphatase ABI2

Structural highlights

3nmv is a 2 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:PYL2, RCAR14, At2g26040, T19L18.15 (ARATH), ABI2, At5g57050, MHM17.19 (ARATH)
Activity:Phosphoprotein phosphatase, with EC number 3.1.3.16
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[PYL2_ARATH] Receptor for abscisic acid (ABA) required for ABA-mediated responses such as stomatal closure and germination inhibition. Inhibits the activity of group-A protein phosphatases type 2C (PP2Cs) when activated by ABA.[1] [2] [P2C77_ARATH] Repressor of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane (Pos), high light stress, response to glucose, seed germination and inhibition of vegetative growth. During the stomatal closure regulation, modulates the inward calcium-channel permeability as well as H(2)O(2) and oxidative burst in response to ABA and dehydration. Controls negatively fibrillin that is involved in mediating ABA-induced photoprotection. May be implicated in ABA content regulation. Involved in acquired thermotolerance of root growth and seedling survival. Required for the Erwinia amylovora harpin-induced (HrpN) drought tolerance. Involved in the hydrotropic response.[3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] [22] [23] [24] [25]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The phytohormone abscisic acid (ABA) functions through a family of fourteen PYR/PYL receptors, which were identified by resistance to pyrabactin, a synthetic inhibitor of seed germination. ABA activates these receptors to inhibit type 2C protein phosphatases, such as ABI1, yet it remains unclear whether these receptors can be antagonized. Here we demonstrate that pyrabactin is an agonist of PYR1 and PYL1 but is unexpectedly an antagonist of PYL2. Crystal structures of the PYL2-pyrabactin and PYL1-pyrabactin-ABI1 complexes reveal the mechanism responsible for receptor-selective activation and inhibition, which enables us to design mutations that convert PYL1 to a pyrabactin-inhibited receptor and PYL2 to a pyrabactin-activated receptor and to identify new pyrabactin-based ABA receptor agonists. Together, our results establish a new concept of ABA receptor antagonism, illustrate its underlying mechanisms and provide a rational framework for discovering novel ABA receptor ligands.

Identification and mechanism of ABA receptor antagonism.,Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE Nat Struct Mol Biol. 2010 Sep;17(9):1102-8. Epub 2010 Aug 22. PMID:20729862[26]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Melcher K, Ng LM, Zhou XE, Soon FF, Xu Y, Suino-Powell KM, Park SY, Weiner JJ, Fujii H, Chinnusamy V, Kovach A, Li J, Wang Y, Li J, Peterson FC, Jensen DR, Yong EL, Volkman BF, Cutler SR, Zhu JK, Xu HE. A gate-latch-lock mechanism for hormone signalling by abscisic acid receptors. Nature. 2009 Dec 3;462(7273):602-8. PMID:19898420 doi:10.1038/nature08613
  2. ↑ Yin P, Fan H, Hao Q, Yuan X, Wu D, Pang Y, Yan C, Li W, Wang J, Yan N. Structural insights into the mechanism of abscisic acid signaling by PYL proteins. Nat Struct Mol Biol. 2009 Dec;16(12):1230-6. Epub 2009 Nov 5. PMID:19893533 doi:10.1038/nsmb.1730
  3. ↑ Leung J, Merlot S, Giraudat J. The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell. 1997 May;9(5):759-71. PMID:9165752 doi:10.1105/tpc.9.5.759
  4. ↑ Finkelstein RR. Maternal Effects Govern Variable Dominance of Two Abscisic Acid Response Mutations in Arabidopsis thaliana. Plant Physiol. 1994 Aug;105(4):1203-1208. PMID:12232276
  5. ↑ de Bruxelles GL, Peacock WJ, Dennis ES, Dolferus R. Abscisic acid induces the alcohol dehydrogenase gene in Arabidopsis. Plant Physiol. 1996 Jun;111(2):381-91. PMID:8787023
  6. ↑ Savoure A, Hua XJ, Bertauche N, Van Montagu M, Verbruggen N. Abscisic acid-independent and abscisic acid-dependent regulation of proline biosynthesis following cold and osmotic stresses in Arabidopsis thaliana. Mol Gen Genet. 1997 Mar 18;254(1):104-9. PMID:9108297
  7. ↑ Pei ZM, Kuchitsu K, Ward JM, Schwarz M, Schroeder JI. Differential abscisic acid regulation of guard cell slow anion channels in Arabidopsis wild-type and abi1 and abi2 mutants. Plant Cell. 1997 Mar;9(3):409-23. PMID:9090884 doi:10.1105/tpc.9.3.409
  8. ↑ Webb AA, Hetherington AM. Convergence of the abscisic acid, CO2, and extracellular calcium signal transduction pathways in stomatal guard cells. Plant Physiol. 1997 Aug;114(4):1557-60. PMID:9276963
  9. ↑ Allen GJ, Kuchitsu K, Chu SP, Murata Y, Schroeder JI. Arabidopsis abi1-1 and abi2-1 phosphatase mutations reduce abscisic acid-induced cytoplasmic calcium rises in guard cells. Plant Cell. 1999 Sep;11(9):1785-98. PMID:10488243
  10. ↑ Arenas-Huertero F, Arroyo A, Zhou L, Sheen J, Leon P. Analysis of Arabidopsis glucose insensitive mutants, gin5 and gin6, reveals a central role of the plant hormone ABA in the regulation of plant vegetative development by sugar. Genes Dev. 2000 Aug 15;14(16):2085-96. PMID:10950871
  11. ↑ Chak RK, Thomas TL, Quatrano RS, Rock CD. The genes ABI1 and ABI2 are involved in abscisic acid- and drought-inducible expression of the Daucus carota L. Dc3 promoter in guard cells of transgenic Arabidopsis thaliana (L.) Heynh. Planta. 2000 May;210(6):875-83. PMID:10872217
  12. ↑ Murata Y, Pei ZM, Mori IC, Schroeder J. Abscisic acid activation of plasma membrane Ca(2+) channels in guard cells requires cytosolic NAD(P)H and is differentially disrupted upstream and downstream of reactive oxygen species production in abi1-1 and abi2-1 protein phosphatase 2C mutants. Plant Cell. 2001 Nov;13(11):2513-23. PMID:11701885
  13. ↑ Merlot S, Gosti F, Guerrier D, Vavasseur A, Giraudat J. The ABI1 and ABI2 protein phosphatases 2C act in a negative feedback regulatory loop of the abscisic acid signalling pathway. Plant J. 2001 Feb;25(3):295-303. PMID:11208021
  14. ↑ Morillon R, Chrispeels MJ. The role of ABA and the transpiration stream in the regulation of the osmotic water permeability of leaf cells. Proc Natl Acad Sci U S A. 2001 Nov 20;98(24):14138-43. Epub 2001 Nov 13. PMID:11707572 doi:10.1073/pnas.231471998
  15. ↑ Guo Y, Xiong L, Song CP, Gong D, Halfter U, Zhu JK. A calcium sensor and its interacting protein kinase are global regulators of abscisic acid signaling in Arabidopsis. Dev Cell. 2002 Aug;3(2):233-44. PMID:12194854
  16. ↑ Takahashi N, Goto N, Okada K, Takahashi H. Hydrotropism in abscisic acid, wavy, and gravitropic mutants of Arabidopsis thaliana. Planta. 2002 Dec;216(2):203-11. Epub 2002 Aug 7. PMID:12447533 doi:10.1007/s00425-002-0840-3
  17. ↑ Allen GJ, Murata Y, Chu SP, Nafisi M, Schroeder JI. Hypersensitivity of abscisic acid-induced cytosolic calcium increases in the Arabidopsis farnesyltransferase mutant era1-2. Plant Cell. 2002 Jul;14(7):1649-62. PMID:12119381
  18. ↑ Becker D, Hoth S, Ache P, Wenkel S, Roelfsema MR, Meyerhoff O, Hartung W, Hedrich R. Regulation of the ABA-sensitive Arabidopsis potassium channel gene GORK in response to water stress. FEBS Lett. 2003 Nov 6;554(1-2):119-26. PMID:14596925
  19. ↑ Fryer MJ, Ball L, Oxborough K, Karpinski S, Mullineaux PM, Baker NR. Control of Ascorbate Peroxidase 2 expression by hydrogen peroxide and leaf water status during excess light stress reveals a functional organisation of Arabidopsis leaves. Plant J. 2003 Feb;33(4):691-705. PMID:12609042
  20. ↑ Stepansky A, Galili G. Synthesis of the Arabidopsis bifunctional lysine-ketoglutarate reductase/saccharopine dehydrogenase enzyme of lysine catabolism is concertedly regulated by metabolic and stress-associated signals. Plant Physiol. 2003 Nov;133(3):1407-15. Epub 2003 Oct 23. PMID:14576281 doi:10.1104/pp.103.026294
  21. ↑ Dong HP, Yu H, Bao Z, Guo X, Peng J, Yao Z, Chen G, Qu S, Dong H. The ABI2-dependent abscisic acid signalling controls HrpN-induced drought tolerance in Arabidopsis. Planta. 2005 Jun;221(3):313-27. Epub 2004 Dec 15. PMID:15599761 doi:10.1007/s00425-004-1444-x
  22. ↑ Larkindale J, Hall JD, Knight MR, Vierling E. Heat stress phenotypes of Arabidopsis mutants implicate multiple signaling pathways in the acquisition of thermotolerance. Plant Physiol. 2005 Jun;138(2):882-97. Epub 2005 May 27. PMID:15923322 doi:10.1104/pp.105.062257
  23. ↑ Verslues PE, Bray EA. Role of abscisic acid (ABA) and Arabidopsis thaliana ABA-insensitive loci in low water potential-induced ABA and proline accumulation. J Exp Bot. 2006;57(1):201-12. Epub 2005 Dec 9. PMID:16339784 doi:10.1093/jxb/erj026
  24. ↑ Yang Y, Sulpice R, Himmelbach A, Meinhard M, Christmann A, Grill E. Fibrillin expression is regulated by abscisic acid response regulators and is involved in abscisic acid-mediated photoprotection. Proc Natl Acad Sci U S A. 2006 Apr 11;103(15):6061-6. Epub 2006 Mar 29. PMID:16571665 doi:0501720103
  25. ↑ Dekkers BJ, Schuurmans JA, Smeekens SC. Interaction between sugar and abscisic acid signalling during early seedling development in Arabidopsis. Plant Mol Biol. 2008 May;67(1-2):151-67. Epub 2008 Feb 17. PMID:18278579 doi:10.1007/s11103-008-9308-6
  26. ↑ Melcher K, Xu Y, Ng LM, Zhou XE, Soon FF, Chinnusamy V, Suino-Powell KM, Kovach A, Tham FS, Cutler SR, Li J, Yong EL, Zhu JK, Xu HE. Identification and mechanism of ABA receptor antagonism. Nat Struct Mol Biol. 2010 Sep;17(9):1102-8. Epub 2010 Aug 22. PMID:20729862 doi:10.1038/nsmb.1887

3nmv, resolution 2.10Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3nmv&oldid=2807458"