Proteopedia

3bwd

Revision as of 11:27, 19 January 2022 by OCA (talk | contribs)

Crystal structure of the plant Rho protein ROP5Crystal structure of the plant Rho protein ROP5

Structural highlights

3bwd is a 1 chain structure with sequence from Arath. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:,
Gene:ARAC6, RAC2, ROP5 (ARATH)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RAC6_ARATH] May be involved in cell polarity control during the actin-dependent tip growth of pollen tubes.[1] [2] Inactive GDP-bound Rho GTPases reside in the cytosol, are found in a complex with Rho GDP-dissociation inhibitors (Rho GDIs), and are released from the GDI protein in order to translocate to membranes upon activation (By similarity).[3] [4]

Evolutionary Conservation

 

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The small G protein ROP5 from the model plant Arabidopsis thaliana was purified and crystallized using the hanging-drop vapour-diffusion method. ROP5 crystals were obtained using PEG 3000 as precipitant and belong to space group P2(1). A data set was collected to 1.53 A resolution using synchrotron radiation at 100 K. A clear molecular-replacement solution was found using ROP4-GDP of the ROP4-GDP-PRONE8 complex as the search model.

Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5.,Thomas C, Berken A Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1070-2. Epub 2007 Nov 30. PMID:18084097[5]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

See Also

References

  1. ↑ Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH. Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol. 1999 Apr 19;145(2):317-30. PMID:10209027
  2. ↑ Li H, Wu G, Ware D, Davis KR, Yang Z. Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiol. 1998 Oct;118(2):407-17. PMID:9765526
  3. ↑ Kost B, Lemichez E, Spielhofer P, Hong Y, Tolias K, Carpenter C, Chua NH. Rac homologues and compartmentalized phosphatidylinositol 4, 5-bisphosphate act in a common pathway to regulate polar pollen tube growth. J Cell Biol. 1999 Apr 19;145(2):317-30. PMID:10209027
  4. ↑ Li H, Wu G, Ware D, Davis KR, Yang Z. Arabidopsis Rho-related GTPases: differential gene expression in pollen and polar localization in fission yeast. Plant Physiol. 1998 Oct;118(2):407-17. PMID:9765526
  5. ↑ Thomas C, Berken A. Purification, crystallization and preliminary X-ray diffraction analysis of the plant Rho protein ROP5. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Dec 1;63(Pt, 12):1070-2. Epub 2007 Nov 30. PMID:18084097 doi:10.1107/S1744309107059672

3bwd, resolution 1.53Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA
Retrieved from "https://proteopedia.openfox.io/wiki/index.php?title=3bwd&oldid=3505157"