1h10
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HIGH RESOLUTION STRUCTURE OF THE PLECKSTRIN HOMOLOGY DOMAIN OF PROTEIN KINASE B/AKT BOUND TO INS(1,3,4,5)-TETRAKISPHOPHATE
OverviewOverview
The products of PI 3-kinase activation, PtdIns(3,4,5)P3 and its immediate, breakdown product PtdIns(3,4)P2, trigger physiological processes, by, interacting with proteins possessing pleckstrin homology (PH) domains. One, of the best characterized PtdIns(3,4,5)P3/PtdIns(3,4)P2 effector proteins, is protein kinase B (PKB), also known as Akt. PKB possesses a PH domain, located at its N terminus, and this domain binds specifically to, PtdIns(3,4,5)P3 and PtdIns(3,4)P2 with similar affinity. Following, activation of PI 3-kinase, PKB is recruited to the plasma membrane by, virtue of its interaction with PtdIns(3,4,5)P3/PtdIns(3,4)P2. PKB is then, activated by the 3-phosphoinositide-dependent pro-tein kinase-1 (PDK1), which like PKB, possesses a PtdIns(3,4,5)P3/PtdIns(3,4)P2 binding PH, domain. Here, we describe the high-resolution crystal structure of the, isolated PH domain of PKB(alpha) in complex with the head group of, PtdIns(3,4,5)P3. The head group has a significantly different orientation, and location compared to other Ins(1,3,4,5)P4 binding PH domains., Mutagenesis of the basic residues that form ionic interactions with the D3, and D4 phosphate groups reduces or abolishes the ability of PKB to, interact with PtdIns(3,4,5)P3 and PtdIns(3,4)P2. The D5 phosphate faces, the solvent and forms no significant interactions with any residue on the, PH domain, and this explains why PKB interacts with similar affinity with, both PtdIns(3,4,5)P3 and PtdIns(3,4)P2.
About this StructureAbout this Structure
1H10 is a Single protein structure of sequence from Homo sapiens with ACE and 4IP as ligands. Structure known Active Site: 1. Full crystallographic information is available from OCA.
ReferenceReference
High-resolution structure of the pleckstrin homology domain of protein kinase b/akt bound to phosphatidylinositol (3,4,5)-trisphosphate., Thomas CC, Deak M, Alessi DR, van Aalten DM, Curr Biol. 2002 Jul 23;12(14):1256-62. PMID:12176338
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