Glycosidase E335A in complex with glucoseGlycosidase E335A in complex with glucose

Structural highlights

5z3f is a 1 chain structure with sequence from Krifd. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:, ,
Gene:Kfla_1896 (KRIFD)
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[IMGH_KRIFD] Involved in the intracellular degradation of the cyclic tetrasaccharide cyclobis-(1-6)-alpha-nigerosyl (CNN) formed extracellularly from starch. Catalyzes the hydrolysis of alpha-1,6-glucosidic linkage from the non-reducing end of isomaltose to yield beta-D-glucose and D-glucose. Can also act on panose and isomaltotriose at a lower rate. It displays low or no activity toward CNN and the general GH15 enzyme substrates such as maltose, soluble starch or dextran.[1]

References

  1. Tagami T, Miyano E, Sadahiro J, Okuyama M, Iwasaki T, Kimura A. Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1-->6)-alpha-nigerosyl. J Biol Chem. 2016 Aug 5;291(32):16438-47. doi: 10.1074/jbc.M116.727305. Epub 2016, Jun 14. PMID:27302067 doi:http://dx.doi.org/10.1074/jbc.M116.727305

5z3f, resolution 1.10Å

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OCA